Wada N, Ide H
Biological Institute, Tohoku University, Sendai, Japan.
Int J Dev Biol. 1994 Jun;38(2):351-6.
To examine differences in the surface properties of limb bud cells, we mixed cells from the progress zone at different stages or from different positions along proximodistal axis of chick and quail wing limb buds. To identify the origin of cells, a chick-specific antibody was used in a mixed culture of chick and quail cells, or cells from one of the stages were labeled with a fluorescent dye, PKH-26. Within 18 hours in mixed culture, cells segregated from each other and formed patches of various sizes. The process zone cells at early developmental stages mixed homogeneously with the cells from proximal region of old limb buds and the progress zone cells at late stages mixed homogeneously with the cells from distal region of the old limb buds. These results suggest that surface properties of cells in progress zone change during limb bud development and vary along the proximodistal and anteroposterior axes of the limb bud and that these differences in surface property may correspond to the positional values for limb pattern formation.
为了研究肢体芽细胞表面特性的差异，我们将来自鸡和鹌鹑翅膀肢体芽不同阶段的进展区或沿近远轴不同位置的细胞进行混合。为了确定细胞的来源，在鸡和鹌鹑细胞的混合培养中使用了鸡特异性抗体，或者用荧光染料PKH-26对其中一个阶段的细胞进行标记。在混合培养的18小时内，细胞彼此分离并形成大小各异的斑块。早期发育阶段的进展区细胞与老肢体芽近端区域的细胞均匀混合，晚期进展区细胞与老肢体芽远端区域的细胞均匀混合。这些结果表明，进展区细胞的表面特性在肢体芽发育过程中发生变化，并沿肢体芽的近远轴和前后轴有所不同，并且这些表面特性的差异可能与肢体模式形成的位置值相对应。
