Mach K E, Fevold H R
Division of Biological Sciences, University of Montana, Missoula 59812.
J Steroid Biochem Mol Biol. 1994 Nov;51(3-4):149-56. doi: 10.1016/0960-0760(94)90088-4.
Stimulation of transcription of the CYP17 gene by ACTH has been demonstrated previously by others using cultures of adrenal tissue from several species. In the present investigations we have demonstrated from measurements in pooled rabbit adrenal glands that after 4 or 6 days of ACTH injections no difference in amounts of CYP17 mRNA per microgram of total adrenal RNA was observed between ACTH and control animals. While the total amount of CYP17 mRNA per adrenal increased 1.4- to 1.7-fold over the injected and non-injected controls due to an increase in total RNA in the ACTH-stimulated adrenals, Western analysis of adrenal microsomal protein demonstrated that the amount of adrenal P450c17 protein, the product of the CYP17 gene, increased over 50-fold. The data show that ACTH is acting at both transcriptional and post-transcriptional loci to increase the amount of rabbit adrenal P450c17 and that the greater effect is post-transcriptional.
此前，其他研究人员利用来自多个物种的肾上腺组织培养物，证实了促肾上腺皮质激素（ACTH）对CYP17基因转录的刺激作用。在本研究中，我们通过对合并的兔肾上腺进行测量发现，注射ACTH 4天或6天后，ACTH处理组与对照组动物每微克肾上腺总RNA中CYP17 mRNA的量没有差异。由于ACTH刺激的肾上腺中总RNA增加，每个肾上腺中CYP17 mRNA的总量比注射和未注射对照组增加了1.4至1.7倍，而对肾上腺微粒体蛋白的蛋白质免疫印迹分析表明，CYP17基因的产物肾上腺P450c17蛋白的量增加了50倍以上。数据表明，ACTH在转录和转录后水平均发挥作用，以增加兔肾上腺P450c17的量，且转录后水平的作用更大。
