Müller W D, Karamfilov T, Fahlbusch B, Vogelsang H, Jäger L
Abteilung für Klinische Immunologie, Friedrich-Schiller-Universität, Jena, Deutschland.
Int Arch Allergy Immunol. 1994 Dec;105(4):391-6. doi: 10.1159/000236789.
Twenty-seven T cell clones (TCC) reactive with group V allergens of Phleum pratense (Phlp V) were established from the peripheral blood of 3 patients allergic to grass pollen. Twenty-four TCC showed the helper cell phenotype CD3+, CD4+, CD8-; the remaining clones were CD3+CD4-CD8+. T cell recognition of Phlp V was exclusively HLA-DR restricted. Many of the Phlp V reactive TCC (19 of 27; 70%) were stimulated additionally by other group V allergens isolated from Lolium perenne, Poa pratensis, and Dactylis glomerata. These data indicate the existence of cross-reacting T cell epitopes among group V allergens of different grasses. The Phlp V triggered cytokine production demonstrated in 13 out of 24 CD4+ TCC a Th2-like pattern (high interleukin 4/gamma-interferon ratios) suggesting group V allergens as important targets of grass pollen specific IgE.
从3名对草花粉过敏患者的外周血中建立了27个与梯牧草V组变应原（Phlp V）反应的T细胞克隆（TCC）。24个TCC表现为辅助性细胞表型CD3 +、CD4 +、CD8 -；其余克隆为CD3 + CD4 - CD8 +。T细胞对Phlp V的识别仅受HLA - DR限制。许多对Phlp V反应的TCC（27个中的19个；70%）还受到从黑麦草、早熟禾和鸭茅中分离出的其他V组变应原的刺激。这些数据表明不同草类的V组变应原之间存在交叉反应性T细胞表位。在24个CD4 + TCC中的13个中显示，Phlp V触发的细胞因子产生呈现出Th2样模式（高白细胞介素4/γ-干扰素比率），提示V组变应原是草花粉特异性IgE的重要靶标。
