Maekawa S, Toriyama M
Graduate School, Division of Science, University of Tokyo, Japan.
Biochem Mol Biol Int. 1994 Jul;33(4):661-8.
Tropomyosin was purified from a gelation product produced in the crude extract of bovine adrenal medulla using several column chromatographies mixed with a boiling treatment. Purified tropomyosin was a mixture of major isoforms with low molecular weights and minor isoforms of high molecular weights. Purified tropomyosin showed a dose-dependent protective effect on actin filaments against the severing activity of adseverin derived from adrenal medulla, suggesting its important role in the regulation of cell shape and structure in vivo.
