羟基硬脂酸对HT29和I407细胞增殖的体外作用。

In vitro effects of hydroxystearic acid on the proliferation of HT29 and I407 cells.

作者信息

Gesmundo N, Casali E, Farruggia G, Spisni A, Masotti L

机构信息

Department of Biochemistry G. Moruzzi, University of Bologna, Italy.

出版信息

Biochem Mol Biol Int. 1994 Jul;33(4):705-12.

PMID:7981658

Abstract

The in vitro effects of hydroxystearic acid on the proliferation of human colon carcinoma cells (HT29) and human embryonic intestine cells (I407) were examined and compared to previous results obtained in murine C108 lung carcinoma cells. The cells were cultured in the presence, or in the absence, of hydroxystearic acid and tested for cell proliferation and viability; the distribution of cells in the cell cycle was evaluated by flow cytometry. Results show that hydroxystearic acid is also an inhibitor of human cell proliferation, and not only of murine C108 cells. Differently from C108 cells, which upon treatment with hydroxystearic acid accumulate in G2-M phases, hydroxystearic acid-treated HT29 cells increase significantly in numbers in G0-G1; I407, embryonic cells used as a control, when treated show only a slight increase in G0-G1.

摘要

研究了羟基硬脂酸对人结肠癌细胞（HT29）和人胚胎肠细胞（I407）增殖的体外作用，并与先前在小鼠C108肺癌细胞中获得的结果进行比较。细胞在有或无羟基硬脂酸的情况下进行培养，并检测细胞增殖和活力；通过流式细胞术评估细胞在细胞周期中的分布。结果表明，羟基硬脂酸不仅是小鼠C108细胞的增殖抑制剂，也是人细胞增殖的抑制剂。与经羟基硬脂酸处理后在G2-M期积累的C108细胞不同，经羟基硬脂酸处理的HT29细胞在G0-G1期数量显著增加；用作对照的胚胎细胞I407经处理后在G0-G1期仅略有增加。