Gadzheva V, Ichimori K, Nakazawa H, Raikov Z
Department of Chemistry and Biochemistry, Medical University, Stara Zagora, Bulgaria.
Free Radic Res. 1994 Sep;21(3):177-86. doi: 10.3109/10715769409056568.
Superoxide scavenging activities (SSA) of newly synthesized spin-labeled nitrosourea and triazene derivatives, and their precursor nitroxides were investigated by the ESR/spin-trapping method using the spin trap 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) and hypoxanthine/xanthine oxidase as the superoxide-generating system. The spin-labeled nitrosoureas, triazenes and their precursor nitroxides exhibited excellent SSA, whereas clinically used nitrosourea and triazene, which do not contain the nitroxide moiety, did not show any SSA. Furthermore, it was deduced that these nitroxides scavenge superoxide by redox cycling between nitroxide and corresponding hydroxylamine.
使用自旋捕集剂5,5 - 二甲基 - 1 - 吡咯啉 - N - 氧化物（DMPO）以及次黄嘌呤/黄嘌呤氧化酶作为超氧化物生成系统，通过电子自旋共振/自旋捕集法研究了新合成的自旋标记亚硝基脲和三氮烯衍生物及其前体氮氧化物的超氧化物清除活性（SSA）。自旋标记的亚硝基脲、三氮烯及其前体氮氧化物表现出优异的SSA，而临床使用的不含氮氧化物部分的亚硝基脲和三氮烯则未显示出任何SSA。此外，据推测这些氮氧化物通过在氮氧化物和相应羟胺之间的氧化还原循环来清除超氧化物。
