Han K C, Shen J R, Ikeuchi M, Inoue Y
Solar Energy Research Group, Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.
FEBS Lett. 1994 Nov 28;355(2):121-4. doi: 10.1016/0014-5793(94)01182-6.
Chemical crosslinking with a zero-length crosslinker, 1-ethyl-3-(3-dimethylaminopropyl) carbodimide hydrochloride, was applied to a cyanobacterial photosystem II complex retaining three extrinsic proteins, the 33 kDa manganese-stabilizing protein, cytochrome (cyt) c-550 and the 12 kDa protein. Three major crosslinked products were obtained in addition to the crosslinked product between the extrinsic 33 kDa and the intrinsic CP47 proteins. They were identified to be: cyt c-550-12 kDa; cyt c-550-12 kDa-33 kDa; D2-cyt c-550-12 kDa. These results indicate that the three extrinsic proteins are closely located with each other in cyanobacterial PSII, supporting the previous proposal that, like the 33 kDa protein; cyt c-550 and the 12 kDa protein are associated with PSII at the lumenal side of thylakoids. The results also suggested that the D2 reaction center protein provides a direct binding site for the extrinsic cyt c-550.
使用零长度交联剂盐酸1-乙基-3-(3-二甲基氨基丙基)碳二亚胺对保留三种外在蛋白（33 kDa锰稳定蛋白、细胞色素（cyt）c-550和12 kDa蛋白）的蓝藻光系统II复合物进行化学交联。除了外在的33 kDa蛋白与内在的CP47蛋白之间的交联产物外，还获得了三种主要的交联产物。它们被鉴定为：cyt c-550-12 kDa；cyt c-550-12 kDa-33 kDa；D2-cyt c-550-12 kDa。这些结果表明，在蓝藻光系统II中，这三种外在蛋白彼此紧密相邻，支持了先前的提议，即与33 kDa蛋白一样，cyt c-550和12 kDa蛋白在类囊体腔侧与光系统II相关联。结果还表明，D2反应中心蛋白为外在的cyt c-550提供了一个直接结合位点。
