Donnelly E, Barnett Y A, McCullough W
Department of Biological and Biomedical Sciences, University of Ulster, Jordanstown, N. Ireland, UK.
FEBS Lett. 1994 Nov 28;355(2):201-4. doi: 10.1016/0014-5793(94)01207-5.
Germinating conidiospores (conidia) of Aspergillus nidulans amino acid-requiring strains are hypersensitive to heat, oxidative stress, UV radiation and chemical mutagens when compared with other strains. They also showed an increased mutation rate. Sensitivity to stress conditions has been correlated with an abnormal RAS/cAMP pathway in mutants of S. cerevisiae. We suggest that the RAS/cAMP pathway is defective in germinating conidia of Aspergillus amino acid auxotrophs and that this is responsible for suppressing DNA repair and conferring sensitivity to oxidative stress and heat shock.
与其他菌株相比，构巢曲霉氨基酸需求菌株的萌发分生孢子（分生孢子）对热、氧化应激、紫外线辐射和化学诱变剂高度敏感。它们还表现出更高的突变率。酿酒酵母突变体对胁迫条件的敏感性与异常的RAS/cAMP途径有关。我们认为，RAS/cAMP途径在曲霉氨基酸营养缺陷型的萌发分生孢子中存在缺陷，这导致了DNA修复的抑制以及对氧化应激和热休克的敏感性。
