Dechesne C J, Rabejac D, Desmadryl G
Laboratoire de Neurophysiologie Sensorielle, Unité INSERM 254, Montpellier, France.
J Comp Neurol. 1994 Aug 22;346(4):517-29. doi: 10.1002/cne.903460405.
Calretinin is a calcium-binding protein of the EF-hand family. It has been previously identified in particular cell types of adult guinea pig, rat, and chinchilla inner ear. Development of calretinin immunoreactivity in the mouse inner ear was investigated from embryonic day 13 (E13) to the adult stage. In the adult mouse vestibule, calretinin immunoreactivity was present in the same structures as described for the rat and guinea pig: the population of afferent fibers forming calyx units and a small number of ganglion neurons. The earliest immunoreactivity was found at E17 in vestibular hair cells (VHCs), then, at E19, in afferent fibers entering the sensory epithelia and in rare ganglion neurons. At postnatal day 4 (P4), a few vestibular nerve fibers and ganglion neurons were reactive. From this stage until P14, immunoreactivity developed in the calyx units and disappeared from VHCs. At P14, immunostaining was adult-like. In the adult mouse cochlea, immunoreactivity was present in the same cell populations as described in the rat: the inner hair cells (IHCs) and most of Corti's ganglion neurons. Calretinin immunoreactivity appeared at E19-P0 in IHCs and ganglion neurons of the basal turn. At P1, outer hair cells (OHCs) of the basal turn were positive. Calretinin immunoreactivity then appeared in IHCs, OHCs, and ganglion neurons of the medial turn, then of the apical turn. At P4, all IHCs and OHCs and most of the ganglion neurons were immunostained. Immunoreactivity gradually disappeared from the OHCs starting at P10 and, at P22, only IHCs and ganglion neurons were positive. The sequences of appearance of calretinin were specific to each cell type of the inner ear and paralleled their respective maturation. Calretinin was transiently expressed in VHCs and OHCs.
钙视网膜蛋白是一种EF手型家族的钙结合蛋白。此前已在成年豚鼠、大鼠和毛丝鼠内耳的特定细胞类型中鉴定出该蛋白。研究了从小鼠胚胎第13天（E13）到成年阶段内耳中钙视网膜蛋白免疫反应性的发育情况。在成年小鼠前庭中，钙视网膜蛋白免疫反应性出现在与大鼠和豚鼠相同的结构中：形成花萼单位的传入纤维群体和少数神经节神经元。最早的免疫反应性在E17时在前庭毛细胞（VHC）中发现，然后在E19时，在进入感觉上皮的传入纤维和罕见的神经节神经元中发现。在出生后第4天（P4），一些前庭神经纤维和神经节神经元具有反应性。从这个阶段到P14，花萼单位中出现免疫反应性，而VHC中的免疫反应性消失。在P14时，免疫染色与成年时相似。在成年小鼠耳蜗中，免疫反应性出现在与大鼠相同的细胞群体中：内毛细胞（IHC）和大多数柯蒂氏神经节神经元。钙视网膜蛋白免疫反应性在E19 - P0时出现在基底转的IHC和神经节神经元中。在P1时，基底转的外毛细胞（OHC）呈阳性。然后钙视网膜蛋白免疫反应性出现在中阶的IHC、OHC和神经节神经元中，接着出现在顶阶。在P4时，所有的IHC和OHC以及大多数神经节神经元都被免疫染色。从P10开始，OHC中的免疫反应性逐渐消失，在P22时，只有IHC和神经节神经元呈阳性。钙视网膜蛋白出现的顺序对于内耳的每种细胞类型都是特定的，并且与其各自的成熟过程平行。钙视网膜蛋白在前庭毛细胞和外毛细胞中短暂表达。
