Trumble T E, Gunlikson R, Parvin D
Department of Orthopaedics, University of Washington, Seattle 98195.
J Orthop Res. 1994 Nov;12(6):844-52. doi: 10.1002/jor.1100120612.
The use of peripheral nerve transplantation in limb reconstruction has been limited by tissue rejection. In order to identify the major histocompatibility antigens involved in tissue rejection, mutant strains of inbred mice, differing from the parent strain (C57BL/6) by either major histocompatibility complex Class I (B6.C-H2bml mice) or Class II (B6.C-H2bml2 mice), were used in models of nerve transplantation. One, 2, and 3 weeks after nerve or skin transplantation, the immune response in the recipient animal was monitored with use of lymphocyte-dependent cytotoxicity and complement-dependent cytotoxicity assays. Skin transplants were used for comparison as the gold standard of a nonvascularized graft with an easily observable success or failure. There was no significant cellular immune response by the lymphocyte-mediated cytotoxicity assay when nerve or skin transplants involved an isolated Class-I or Class-II mismatch, but there was a significant response 2 weeks after transplantations across a combined Class-I and Class-II barrier for nerve (p < 0.04) or skin (p < 0.03). An antibody response to the grafts occurred for both skin and nerve transplants but only when a combined barrier was involved. This preliminary study, using a mouse model, suggests that nerve transplantation-may be performed without systemic evidence of rejection with only a partial cross match of the major histocompatibility complexes, thus decreasing the complexity of tissue typing necessary for tissue banking.
肢体重建中周围神经移植的应用一直受到组织排斥的限制。为了确定参与组织排斥的主要组织相容性抗原，在神经移植模型中使用了近交系小鼠的突变株，这些突变株在主要组织相容性复合体I类（B6.C-H2bm1小鼠）或II类（B6.C-H2bm12小鼠）方面与亲代株（C57BL/6）不同。在神经或皮肤移植后1、2和3周，通过淋巴细胞依赖性细胞毒性和补体依赖性细胞毒性试验监测受体动物的免疫反应。皮肤移植用作对照，作为非血管化移植物成功或失败易于观察的金标准。当神经或皮肤移植涉及孤立的I类或II类错配时，淋巴细胞介导的细胞毒性试验未显示明显的细胞免疫反应，但在跨越I类和II类联合屏障进行神经（p<0.04）或皮肤（p<0.03）移植后2周出现了明显反应。皮肤和神经移植均出现了针对移植物的抗体反应，但仅在涉及联合屏障时出现。这项使用小鼠模型的初步研究表明，仅进行主要组织相容性复合体的部分交叉配型，神经移植可能在无全身排斥证据的情况下进行，从而降低了组织库所需的组织分型复杂性。
