Ploski R, Mellbye O J, Rønningen K S, Førre O, Thorsby E
Institute of Transplantation Immunology, Univeristy of Oslo, Rikshospitalet, Norway.
J Rheumatol. 1994 Aug;21(8):1397-402.
To study the role of HLA class II genes in conferring susceptibility to seronegative vs seropositive rheumatoid arthritis (RA).
The distribution of DRB1 and DPB1 alleles in 33 seronegative, 20 weakly seropositive, 79 distinctly seropositive patients with RA, and in 181 healthy controls was determined by genomic typing: IgM rheumatoid factor (RF) seronegativity was ascertained in Waalers' latex, and ELISA tests.
DRB10101/2 (encoding variants of DR1) was associated with weakly seropositive RA (and possibly seronegative RA) but not the clearly seropositive disease. In contrast DRB10401 and DRB10408 (encoding the Dw4 and Dw14.2 variants of DR4, respectively) were associated with clearly seropositive RA but were not increased among patients having weakly seropositive or seronegative RA. DRB10404 (encoding the Dw14.1 variant of DR4) was increased to some extent in all examined RA subsets. The distribution of DPB1 alleles in the patients was as in the controls.
Seronegative and weakly seropositive RA differ from clearly seropositive RA in HLA class II associations.
研究人类白细胞抗原（HLA）Ⅱ类基因在血清阴性与血清阳性类风湿关节炎（RA）易感性中的作用。
采用基因分型法确定33例血清阴性、20例弱阳性、79例强阳性RA患者以及181例健康对照者中DRB1和DPB1等位基因的分布情况：通过瓦勒氏乳胶试验和酶联免疫吸附测定（ELISA）检测确定IgM类风湿因子（RF）血清阴性。
DRB10101/2（编码DR1变体）与弱阳性RA（可能还有血清阴性RA）相关，但与强阳性RA无关。相比之下，DRB10401和DRB10408（分别编码DR4的Dw4和Dw14.2变体）与强阳性RA相关，但在弱阳性或血清阴性RA患者中未增加。DRB10404（编码DR4的Dw14.1变体）在所有检测的RA亚组中均有一定程度的增加。患者中DPB1等位基因的分布与对照组相同。
血清阴性和弱阳性RA在HLAⅡ类关联方面与强阳性RA不同。
