基底大细胞和脑桥胆碱能神经元共同表达成纤维细胞生长因子受体信使核糖核酸。

Basal magnocellular and pontine cholinergic neurons coexpress FGF receptor mRNA.

作者信息

Yoshida S, Lin L P, Chen Z L, Momota Y, Kato K, Tanaka T, Wanaka A, Shiosaka S

机构信息

Nara Institute of Science and Technology (NAIST), Japan.

出版信息

Neurosci Res. 1994 Jul;20(1):35-42. doi: 10.1016/0168-0102(94)90020-5.

DOI:10.1016/0168-0102(94)90020-5

PMID:7984339

Abstract

By in situ hybridization histochemistry, fibroblast growth factor receptor gene (flg)-expressing neurons were newly identified in the basal magnocellular nuclei (the vertical and horizontal limbs of the diagonal band, and Meynert's nucleus). The present study also confirmed flg localization in the laterodorsal tegmental nucleus and the pedunculopontine tegmental nucleus of the pons. Immuno- and in situ hybridization histochemistry on the same sections demonstrated that choline acetyltransferase and flg were colocalized in single neurons of the diagonal band, Meynert's nucleus and the pontine tegmental areas. The results suggest that a significant number of the basal magnocellular and a majority of the mesopontine cholinergic neurons are directly affected by fibroblast growth factors (FGF) via FGF receptor gene.

摘要

通过原位杂交组织化学技术，在基底大细胞核（斜角带垂直和水平支以及迈内特核）中首次鉴定出表达成纤维细胞生长因子受体基因（flg）的神经元。本研究还证实了flg在脑桥背外侧被盖核和脑桥脚被盖核中的定位。对同一切片进行免疫组织化学和原位杂交组织化学显示，胆碱乙酰转移酶和flg共定位于斜角带、迈内特核和脑桥被盖区的单个神经元中。结果表明，大量基底大细胞和大多数脑桥中脑胆碱能神经元通过成纤维细胞生长因子（FGF）受体基因直接受到成纤维细胞生长因子（FGF）的影响。