Senanayake P D, Moriguchi A, Kumagai H, Ganten D, Ferrario C M, Brosnihan K B
Hypertension Center, Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, NC 27105.
Peptides. 1994;15(5):919-26. doi: 10.1016/0196-9781(94)90051-5.
We determined the levels of angiotensin I (ANG I), angiotensin II (ANG II), and the heptapeptide angiotensin(1-7) [ANG(1-7)] in the blood and brain of female Hannover Sprague-Dawley (SD) and transgenic hypertensive rats [mRen-2]27 by radioimmunoassay and high performance liquid chromatography. Hypertension was accompanied by higher plasma concentrations of ANG II, no statistical changes in ANG(1-7), and no differences in plasma ANG I levels. In the hypothalamus of transgenic rats, concentrations of ANG II and ANG(1-7) averaged 827% and 168% above values in SD rats (p < 0.005) whereas both ANG I and ANG II increased in the medulla oblongata. The data showed that the established phase of hypertension in rats harboring the mouse Ren-2 gene is associated with overexpression of the renin-angiotensin system in brain regions participating in the endocrine regulation of blood pressure.
我们通过放射免疫分析和高效液相色谱法测定了雌性汉诺威-斯普拉格-道利（SD）大鼠和转基因高血压大鼠[mRen-2]27血液及大脑中血管紧张素I（ANG I）、血管紧张素II（ANG II）以及七肽血管紧张素（1-7）[ANG(1-7)]的水平。高血压伴随着血浆ANG II浓度升高，ANG(1-7)无统计学变化，血浆ANG I水平无差异。在转基因大鼠的下丘脑，ANG II和ANG(1-7)的浓度平均比SD大鼠的值高出827%和168%（p<0.005），而延髓中的ANG I和ANG II均增加。数据表明，携带小鼠Ren-2基因的大鼠高血压的既定阶段与参与血压内分泌调节的脑区肾素-血管紧张素系统的过表达有关。
