Fóris G
Acta Microbiol Acad Sci Hung. 1976;23(3):271-6.
In rats weighing 100 g, intravenously administered Trypanosoma equiperdum organisms started logarithmic growth immediately, whereas in rats weighing 150-300 g growth started after a lag phase of 0.7-3.1 hr. The lag phase lasted for 15-17 hr when the rats were pretreated serially with endotoxins and this time course was not modified by changes either in the host's body weight or in the germ count of the inocula. Maximum resistance to infection was achieved with gradual doses of the endotoxin given at 48 hr intervals on 6 occasions. The total dose of endotoxin was fourfold of its LD50. Additional doses failed further to increase the resistance. Serial passages of the strain in rats pretreated with endotoxin disclosed that the trypanosomes growing after the lag phase had developed resistance to the factor responsible for the lag phase. Thus the method does not allow a quantitative estimation of resistance induced by serial endotoxin treatment, yet it represents a rapid and simple procedure in every case in which qualitative assessment is sufficient.
在体重100克的大鼠中，静脉注射的马媾疫锥虫生物体立即开始对数生长，而在体重150 - 300克的大鼠中，生长在0.7 - 3.1小时的延迟期后开始。当大鼠连续用内毒素预处理时，延迟期持续15 - 17小时，并且这个时间进程不会因宿主体重或接种物菌数的变化而改变。通过每隔48小时分6次逐渐给予内毒素剂量，可实现对感染的最大抵抗力。内毒素的总剂量是其半数致死量的四倍。额外的剂量未能进一步提高抵抗力。在用内毒素预处理的大鼠中对该菌株进行连续传代发现，在延迟期后生长的锥虫已对导致延迟期的因素产生了抗性。因此，该方法不允许对连续内毒素处理诱导的抗性进行定量估计，但在定性评估足够的每种情况下，它都代表了一种快速且简单的程序。
