Santamarina Mijares A, García Avila I, González Broche R
Instituto de Medicina Tropical Pedro Kourí.
Rev Cubana Med Trop. 1993;45(2):128-31.
Field tests were carried out with the nematode parasite Romanomermis iyengari (Welch, 1964) to fight 3 species of mosquito larvae Anopheles albimanus (Wiedeman, 1821); Culex nigripalpus (Theobald, 1901); and Culex quinquefasciatus (Say, 1823) in three ecologically different natural mosquito reservoirs. The release of the nematode embryos (infective stage) in the above-mentioned reservoirs was carried out with a Holder-planta 5 manual sprayer, at 2 atm, and an application dose of 1000 parasite embryos/m2. The parasite embryos were obtained from 6 nematode cultures, soaked in distilled water. Results showed that mosquito larvae were parasitized by R. iyengari as follows: 100% of the A. albimanus and 85% of the C. nigripalpus, in the first reservoir; 85% of the C. nigripalpus, in the second reservoir; and 80% of the C. nigripalpus and 75% of the C. quinquefasciatus in the third one. Thus, the potential use of the nematode parasite Romanomermis iyengari as a biological control agent of mosquito larvae was shown.
利用线虫寄生虫伊氏罗索线虫（罗曼诺梅尔斯·伊扬加里，韦尔奇，1964年）在三个生态环境不同的天然蚊虫滋生地对三种蚊虫幼虫进行了实地试验，这三种蚊虫幼虫分别是白纹伊蚊（维德曼，1821年）、黑胸库蚊（西奥博尔德，1901年）和致倦库蚊（赛伊，1823年）。使用Holder - planta 5手动喷雾器在上述滋生地释放线虫胚胎（感染期），压力为2个大气压，施用量为每平方米1000个寄生虫胚胎。寄生虫胚胎取自6个线虫培养物，浸泡在蒸馏水中。结果表明，伊氏罗索线虫对蚊虫幼虫的寄生情况如下：在第一个滋生地，100%的白纹伊蚊和85%的黑胸库蚊被寄生；在第二个滋生地，85%的黑胸库蚊被寄生；在第三个滋生地，80%的黑胸库蚊和75%的致倦库蚊被寄生。因此，显示出线虫寄生虫伊氏罗索线虫作为蚊虫幼虫生物防治剂的潜在用途。
