Carmine T C, Bruchelt G, Hahn T, Niethammer D
Children's University Hospital Tübingen, Germany.
J Biolumin Chemilumin. 1994 Jul-Aug;9(4):267-72. doi: 10.1002/bio.1170090404.
A sensitive peroxidase-dependent luminol-enhanced chemiluminescence (ECL) assay for determination of hydrogen peroxide (H2O2) generation by tumour cells was established. This test system allows determination of H2O2 in concentrations as low as 25 pmol (50 nmol/L) and yields results which are comparable to those obtained using a less sensitive photometric method and a previously described scopoletin fluorescence assay. After 3 h incubation time 10(4) SK-N-SH neuroblastoma cells released 60 +/- 5 pmol H2O2 in the supernatant and this level was significantly (p < 0.025) increased by about 70% in the presence of 5 pmol (100 ng/mL) recombinant tumour necrosis factor alpha (TNF alpha). In contrast, H2O2 production was slightly reduced by TNF alpha at a very low concentration of 0.5 fmol (0.01 ng/mL).
建立了一种灵敏的依赖过氧化物酶的鲁米诺增强化学发光（ECL）测定法，用于测定肿瘤细胞产生的过氧化氢（H2O2）。该测试系统能够测定低至25皮摩尔（50纳摩尔/升）浓度的H2O2，其结果与使用灵敏度较低的光度法和先前描述的 scopoletin 荧光测定法获得的结果相当。孵育3小时后，10(4)个SK-N-SH神经母细胞瘤细胞在上清液中释放出60±5皮摩尔的H2O2，在存在5皮摩尔（100纳克/毫升）重组肿瘤坏死因子α（TNFα）的情况下，该水平显著（p<0.025）增加约70%。相比之下，在极低浓度0.5飞摩尔（0.01纳克/毫升）的TNFα作用下，H2O2的产生略有减少。
