人平滑肌自身抗体与胎儿神经胶质细胞及其前体细胞的反应。

Reaction of human smooth muscle autoantibody with foetal glial cells and their precursors.

作者信息

Toh B H, Muller H K, Cauchi M N

出版信息

Aust J Exp Biol Med Sci. 1976 Oct;54(5):423-30. doi: 10.1038/icb.1976.42.

DOI:10.1038/icb.1976.42

Abstract

Tissue culture monolayers of dissociated human and rat embryonic brains were examined by indirect immunoflurescence with human serum containing smooth muscle antibody. Flat, epithelioid cells showed staining of a fine, filamentous network in the cell body while multipolar cells showed diffuse cytoplasmic fluorescence of the cell body and processes. Both cell types were identified as glial cells by silver stains. Specificity of the immunofluorescent staining reaction was established by failure to obtain staining with normal serum, with smooth muscle antibody serum neutralized by homogenates of smooth muscle or by extracts contining actin-like protein derived from smooth muscle. Reacitivity of of embryonic glial cells with smooth muscle antibody indicates the presence of actin-like proteins which are probably located in cytoplasmic microfilaments.

摘要

用人血清中的平滑肌抗体通过间接免疫荧光法检测解离的人及大鼠胚胎脑组织的组织培养单层。扁平上皮样细胞在细胞体内显示出精细的丝状网络染色，而多极细胞在细胞体和突起中显示出弥漫性细胞质荧光。两种细胞类型经银染鉴定为神经胶质细胞。通过用正常血清未获得染色结果、用平滑肌匀浆中和平滑肌抗体血清或用含有源自平滑肌的肌动蛋白样蛋白的提取物中和平滑肌抗体血清，确定了免疫荧光染色反应的特异性。胚胎神经胶质细胞与平滑肌抗体的反应性表明存在可能位于细胞质微丝中的肌动蛋白样蛋白。