Casteels M, Croes K, Van Veldhoven P P, Mannaerts G P
Katholieke Universiteit Leuven, Afdeling Farmacologie, Belgium.
Biochem Pharmacol. 1994 Nov 16;48(10):1973-5. doi: 10.1016/0006-2952(94)90596-7.
The production of CO2 and formate in isolated rat hepatocytes incubated in the presence of 3-methyl[1-14C]margaric acid was investigated. Production rates of formate were approximately 4-fold lower than those of CO2. Aminotriazole (3-amino-1, 2, 4-triazole), an irreversible inhibitor of catalase, potently suppressed alpha-oxidation of 3-methylmargaric acid, whereas beta-oxidation of palmitate, 2-methylpalmitate and trihydroxycoprostanic acid and conversion of exogenously added formate to CO2 were not or only slightly affected. This shows that aminotriazole is not only an inhibitor of catalase, but also of alpha-oxidation of 3-methyl-substituted fatty acids.
研究了在3-甲基[1-¹⁴C]十七烷酸存在下孵育的离体大鼠肝细胞中二氧化碳和甲酸的生成情况。甲酸的生成速率比二氧化碳的生成速率低约4倍。氨基三唑（3-氨基-1,2,4-三唑），一种过氧化氢酶的不可逆抑制剂，能有效抑制3-甲基十七烷酸的α-氧化，而棕榈酸、2-甲基棕榈酸和三羟基胆甾烷酸的β-氧化以及外源添加的甲酸向二氧化碳的转化未受影响或仅受到轻微影响。这表明氨基三唑不仅是过氧化氢酶的抑制剂，也是3-甲基取代脂肪酸α-氧化的抑制剂。
