豚鼠心脏钠钙交换体的分子克隆与功能表达

Molecular cloning and functional expression of the guinea pig cardiac Na(+)-Ca2+ exchanger.

作者信息

Tsuruya Y, Bersohn M M, Li Z, Nicoll D A, Philipson K D

机构信息

Department of Physiology, UCLA School of Medicine 90024-1760.

出版信息

Biochim Biophys Acta. 1994 Nov 23;1196(1):97-9. doi: 10.1016/0005-2736(94)90301-8.

DOI:10.1016/0005-2736(94)90301-8

PMID:7986817

Abstract

The cDNA of the guinea pig cardiac Na(+)-Ca2+ exchanger was cloned from a lambda ZAP cDNA library. The deduced sequence of the protein corresponds to 970 amino acids and is 98% identical to the canine cardiac exchanger. The leader peptide region shows substantial variation among species. The cloned cDNA can induce Na(+)-Ca2+ exchange activity when in vitro transcribed cRNA is injected into Xenopus laevis oocytes.

摘要

豚鼠心脏钠钙交换体的互补脱氧核糖核酸（cDNA）是从λZAP cDNA文库中克隆出来的。该蛋白质的推导序列对应970个氨基酸，与犬心脏交换体的序列有98%的同源性。前导肽区域在不同物种间存在显著差异。当将体外转录的cRNA注射到非洲爪蟾卵母细胞中时，克隆得到的cDNA能够诱导钠钙交换活性。