Jorge-Herrero E, Fernández P, de la Torre N, Escudero C, García-Páez J M, Buján J, Castillo-Olivares J L
Service of Experimental Surgery, Clinica Puerta de Hierro, Madrid, Spain.
Biomaterials. 1994 Aug;15(10):815-20. doi: 10.1016/0142-9612(94)90036-1.
Since the development of cardiac prostheses, numerous chemical treatments have been assayed to prevent the process of their mineralization, causing 60% of the failures. The effect of the extraction of lipids from the tissue employed in porcine valves is assessed in a model of subcutaneous implantation in rats. Tissue from aortic and pulmonary porcine valves was treated with chloroform-methanol and 0.625% glutaraldehyde and was implanted into young rats for periods of 21 and 60 d. The calcium accumulated was then quantified by atomic absorption. The effectiveness of this treatment is demonstrated by the detection of much lower calcium values than in the control group. For aortic valve tissue, the values obtained were 40.5 and 188.1 micrograms Ca2+/mg dry weight of tissue for implantation times of 21 and 60 d, respectively, versus 5.48 and 1.4 micrograms Ca2+/mg dry weight of tissue for the same tissue treated with chloroform-methanol. The values obtained with pulmonary valve tissue were very similar: 72.46 and 108.06 micrograms Ca2+/mg dry weight tissue versus 0.67 and 0.80 micrograms Ca2+/mg dry weight tissue for implantation periods of 21 and 60 d, respectively. Thus, phospholipids may be totally or partially responsible for the calcification of the porcine valve tissue employed in the construction of cardiac bioprostheses.
自心脏假体研发以来，人们尝试了多种化学处理方法来防止其矿化过程，因为矿化会导致60%的失败案例。在大鼠皮下植入模型中评估了从猪瓣膜所用组织中提取脂质的效果。用氯仿 - 甲醇和0.625%戊二醛处理猪主动脉瓣和肺动脉瓣组织，然后将其植入幼鼠体内21天和60天。随后通过原子吸收法定量累积的钙。与对照组相比，检测到的钙值低得多，证明了这种处理方法的有效性。对于主动脉瓣组织，植入21天和60天时，每毫克干重组织中获得的钙值分别为40.5和188.1微克Ca2 +，而用氯仿 - 甲醇处理的相同组织每毫克干重组织中的钙值分别为5.48和1.4微克Ca2 +。肺动脉瓣组织获得的值非常相似：植入21天和60天时，每毫克干重组织中分别为72.46和108.06微克Ca2 +，而处理后的每毫克干重组织中分别为0.67和0.80微克Ca2 +。因此，磷脂可能完全或部分导致用于构建心脏生物假体的猪瓣膜组织钙化。
