Murphy J P, Trowern A R, Duggleby C J
Division of Biotechnology, PHLS Centre for Applied Microbiology and Research, Salisbury, Wiltshire, UK.
DNA Seq. 1994;4(4):259-65. doi: 10.3109/10425179409020850.
A gene bank of Peptostreptococcus magnus DNA was established using an E. coli host-vector system. Western blot analysis identified a clone expressing protein L which bound to the light chain of human immunoglobulins. DNA sequence determination and analysis revealed an open reading frame of 992 amino acids, giving a theoretical secreted protein of 106 kD with a pl of 4.67.
利用大肠杆菌宿主 - 载体系统建立了巨大消化链球菌DNA基因文库。蛋白质免疫印迹分析鉴定出一个表达与人类免疫球蛋白轻链结合的蛋白L的克隆。DNA序列测定和分析揭示了一个992个氨基酸的开放阅读框，预测分泌蛋白为106 kD，等电点为4.67。
