如何在恒化器中确定生长速率的控制。利用代谢控制分析解决这一悖论。

How to determine control of growth rate in a chemostat. Using metabolic control analysis to resolve the paradox.

作者信息

Snoep J L, Jensen P R, Groeneveld P, Molenaar D, Kholodenko B N, Westerhoff H V

机构信息

University of Florida, Dept. of Microbiology and Cell Science, Gainesville 32611-0330.

出版信息

Biochem Mol Biol Int. 1994 Aug;33(5):1023-32.

PMID:7987249

Abstract

The chemostat makes it possible to study microbial physiology at steady state. However, because growth rate in a chemostat is set by the experimenter, it seems impossible to employ the chemostat to study the control of microbial growth by processes within the microorganism. In this paper we show how, paradoxically, one can determine control of growth rate, of growth yield and of other fluxes in a chemostat. We develop metabolic control analysis for the chemostat. This analysis does not depend on the particular way in which specific growth rate varies with the concentration of the growth limiting substrate.

摘要

恒化器使在稳态下研究微生物生理学成为可能。然而，由于恒化器中的生长速率由实验者设定，因此似乎不可能利用恒化器来研究微生物内部过程对微生物生长的控制。在本文中，我们将反常地展示如何在恒化器中确定生长速率、生长产量及其他通量的控制。我们为恒化器开发了代谢控制分析方法。该分析并不依赖于比生长速率随生长限制底物浓度变化的具体方式。