Tebbey P W, Van Cleave S, Buttke T M
Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, North Carolina 27858-4354.
Biochem Mol Biol Int. 1994 Aug;33(5):991-1000.
Chronic exposure of BW5147 cells to arachidonic acid results in a complete inhibition of stearoyl-CoA desaturase 2 gene expression. Following removal of the fatty acid, gene transcription increased with a kinetic profile similar to the observed increases in both stearoyl-CoA desaturase 2 mRNA and unsaturated fatty acid synthesis. During the time period when stearoyl-CoA desaturase 2 transcription and unsaturated fatty acid synthesis increased (6-12 h), there was only a minimal depletion (approximately 2%) of the total phospholipid arachidonic acid-content. By contrast, during the same period there was a specific depletion of arachidonic acid from phosphatidylcholine, but the proportion of arachidonic acid in other phospholipid species did not change. Thus, changes in the fatty acid content of phosphatidylcholine may be of particular importance in the genetic regulation of endogenous lipid synthesis.
BW5147细胞长期暴露于花生四烯酸会导致硬脂酰辅酶A去饱和酶2基因表达完全受到抑制。去除脂肪酸后，基因转录增加，其动力学特征类似于观察到的硬脂酰辅酶A去饱和酶2 mRNA和不饱和脂肪酸合成的增加。在硬脂酰辅酶A去饱和酶2转录和不饱和脂肪酸合成增加的时间段（6 - 12小时）内，总磷脂花生四烯酸含量仅出现极小的消耗（约2%）。相比之下，在同一时期，花生四烯酸从磷脂酰胆碱中特异性消耗，但其他磷脂种类中花生四烯酸的比例没有变化。因此，磷脂酰胆碱脂肪酸含量的变化在内源脂质合成的基因调控中可能尤为重要。
