Grobet L
Service de Génétique factorielle et moléculaire, faculté de Médecine vétérinaire, Université de Liège.
Bull Mem Acad R Med Belg. 1993;148(10):345-56.
Different contributions to the development and applications of genomic diagnosis methods in domestic animals are reported. DNA fingerprints were used to solve parentage disputes in different species, and to reveal leucochimerism in bovine dizygotic twins. In cattle, an embryo sexing method and a freemartinism diagnosis assay were also developed. The search for molecular markers of the MH (muscular hypertrophy) and R (rouan) bovine genes is described. Prion protein gene polymorphisms are investigated in the framework of a research on the genetics of bovine spongiform encephalopathies. The mutation responsible for the autosomal recessive lethal condition BLAD (bovine leucocyte adhesion deficiency) in Holstein cattle is found in our country, and the carries can be detected by a molecular method. The mutation responsible for the halothane sensitivity in pig can also be detected, what is done to control efficiently the introgression of the stress resistance allele in a Piétrain strain.
本文报道了在家畜基因组诊断方法的开发和应用方面的不同贡献。DNA指纹图谱被用于解决不同物种的亲子关系纠纷，并揭示牛双合子双胞胎中的白细胞嵌合体现象。在牛中，还开发了一种胚胎性别鉴定方法和一种双胎母犊不育诊断检测方法。文中描述了对牛的MH（肌肉肥大）和R（柔安）基因分子标记的搜索。在牛海绵状脑病遗传学研究的框架内，对朊病毒蛋白基因多态性进行了研究。我国发现了荷斯坦牛常染色体隐性致死疾病BLAD（牛白细胞粘附缺陷）的致病突变，并且可以通过分子方法检测携带者。猪中导致氟烷敏感性的突变也可以被检测到，这有助于有效控制皮特兰品系中抗应激等位基因的渗入。
