Sasaki T, Song J, Koga-Ban Y, Matsui E, Fang F, Higo H, Nagasaki H, Hori M, Miya M, Murayama-Kayano E
Rice Genome Research Program, National Institute of Agrobiological Resources/Institute of Society for Technoinnovation of Agriculture, Forestry and Fisheries, Ibaraki, Japan.
Plant J. 1994 Oct;6(4):615-24. doi: 10.1046/j.1365-313x.1994.6040615.x.
A large-scale sequence analysis of rice cDNA was performed for a library from rice callus cultured in a medium containing 1 p.p.m. of 2,4-dichlorophenoxyacetic acid. Random sequencing of 2778 cDNA clones generated 2259 non-redundant expressed sequence tags (ESTs). The strategy of sequencing cDNAs can yield quickly a large number of novel genes. After translation, 690 sequences showed a significant amino acid sequence similarity to sequences already known from PIR. The source of known proteins ranged from bacteria to human. In this report, the non-redundant set of 280 identified ESTs is analyzed in detail.
对在含有百万分之一2,4 - 二氯苯氧乙酸的培养基中培养的水稻愈伤组织文库进行了大规模水稻cDNA序列分析。对2778个cDNA克隆进行随机测序产生了2259个非冗余表达序列标签（EST）。cDNA测序策略能够快速产生大量新基因。翻译后，690个序列显示出与蛋白质信息资源（PIR）中已知序列有显著的氨基酸序列相似性。已知蛋白质的来源从细菌到人类。在本报告中，对280个已鉴定的非冗余EST集合进行了详细分析。
