Liang D, Chow D, White C
Department of Pharmaceutics, University of Houston, Texas Medical Center 77030.
J Chromatogr B Biomed Appl. 1994 Jun 17;656(2):460-5. doi: 10.1016/s0378-4347(94)80109-6.
A rapid, sensitive and reproducible high-performance liquid chromatographic (HPLC) assay for cefazolin in rat tissues was developed. Tissue samples were homogenized in distilled water, acidified with 8.5% phosphoric acid, and centrifuged. Cefazolin was isolated from the supernatant by solid-phase extraction on C18 cartridges. The eluate containing cefazolin and internal standard, cephalexin, was injected onto a reversed-phase C18 column and eluted with a mobile phase of 23% methanol in 0.02 M sodium phosphate monobasic (pH 5.0) and detected with UV absorbance at 270 nm. Recoveries of cefazolin were 33.7 +/- 2.5%, 45.4 +/- 2.1%, and 42.9 +/- 1.0% from liver, spleen and lung, respectively. The calibration curves for cefazolin were established at 0.5-1500 micrograms/g in spleen, 0.1-250 micrograms/g in liver and 0.1-75 micrograms/g in lung. The assay was reproducible with within-day and between-day variations of 1-2 and 1-4%, respectively. Application of the assay for tissue distribution of cefazolin in liposomal targeting study was demonstrated.
建立了一种快速、灵敏且可重复的高效液相色谱（HPLC）法，用于测定大鼠组织中的头孢唑林。将组织样品在蒸馏水中匀浆，用8.5%磷酸酸化，然后离心。通过C18柱固相萃取从上层清液中分离出头孢唑林。将含有头孢唑林和内标头孢氨苄的洗脱液注入反相C18柱，用0.02 M磷酸二氢钠（pH 5.0）中含23%甲醇的流动相洗脱，并在270 nm处用紫外吸收进行检测。头孢唑林在肝脏、脾脏和肺中的回收率分别为33.7±2.5%、45.4±2.1%和42.9±1.0%。头孢唑林的校准曲线在脾脏中为0.5 - 1500微克/克、肝脏中为0.1 - 250微克/克、肺中为0.1 - 75微克/克范围内建立。该方法具有可重复性，日内和日间变化分别为1 - 2%和1 - 4%。证明了该方法在脂质体靶向研究中用于头孢唑林组织分布的应用。
