Pykett M J, Murphy M, Harnish P R, George D L
Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia 19104-6145.
Cancer Res. 1994 Dec 15;54(24):6340-3.
To better understand the molecular mechanisms responsible for meningioma tumorigenesis we previously utilized subtractive hybridization protocols to identify genes the expression or structure of which is altered in these common brain tumors. Here we show that a CA dinucleotide repeat element present in one complementary DNA isolated by this approach has undergone a contraction in size in a meningioma cell line. Extension of this initial observation has revealed widespread genetic alterations affecting simple repeat sequences in this and other meningiomas. These data indicate that genetic instability may play a previously unrecognized role in the etiology of meningiomas.
为了更好地理解脑膜瘤发生的分子机制，我们之前利用消减杂交技术来鉴定在这些常见脑肿瘤中表达或结构发生改变的基因。在此我们表明，通过这种方法分离出的一个互补DNA中存在的CA二核苷酸重复元件在一个脑膜瘤细胞系中发生了大小收缩。对这一初步观察结果的扩展研究揭示了广泛的基因改变，这些改变影响了该脑膜瘤及其他脑膜瘤中的简单重复序列。这些数据表明，基因不稳定性可能在脑膜瘤的病因学中发挥了之前未被认识到的作用。
