Liu S Q, Shiba R, Kim B S, Saijo K, Ohno T
RIKEN Cell Bank, Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan.
Cancer Immunol Immunother. 1994 Nov;39(5):279-85. doi: 10.1007/BF01519979.
Tumor-specific human cytotoxic T lymphocytes (CTL) were induced by co-culturing peripheral blood mononuclear cells with X-ray-irradiated human lung squamous carcinoma cells, SQ-5, in the medium supplemented with interleukin(IL)-1, IL-2, IL-4 and IL-6, and 5% autologous plasma for 3 or 5 days. The CTL grew in serum/plasma-free medium containing these four interleukins and 0.5% bovine serum albumin for over a month and maintained killing activity of target cells within 48 h at an effector/target ratio of 1.25. Their growth was essentially dependent on the target SQ-5 cells, which were renewed every 5 days. Under these conditions, IL-4 and IL-6 could be omitted. When anti-CD3 monoclonal antibody was added to the serum/plasma-free medium supplemented with IL-1 and IL-2, the target tumor cells were not required to maintain the specific killing activity of the CTL. A large number of CTL (10(11)) were obtained in 35 days.
通过将外周血单个核细胞与经X射线照射的人肺鳞癌细胞SQ - 5在补充有白细胞介素（IL）-1、IL - 2、IL - 4和IL - 6以及5%自体血浆的培养基中共同培养3天或5天，诱导出肿瘤特异性人细胞毒性T淋巴细胞（CTL）。CTL在含有这四种白细胞介素和0.5%牛血清白蛋白的无血清/无血浆培养基中生长超过一个月，并在效应细胞/靶细胞比例为1.25时，在48小时内维持对靶细胞的杀伤活性。它们的生长基本上依赖于每5天更新一次的靶细胞SQ - 5。在这些条件下，可以省略IL - 4和IL - 6。当将抗CD3单克隆抗体添加到补充有IL - 1和IL - 2的无血清/无血浆培养基中时，维持CTL的特异性杀伤活性不需要靶肿瘤细胞。在35天内获得了大量的CTL（10¹¹）。
