Chang B V, Chen K S, Yuan S Y
Department of Microbiology, Soochow University, Taipei, Taiwan, Republic of China.
Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1993 Nov;26(4):139-50.
In this study, we investigated the potential for reductive dechlorination of chlorophenols by municipal sewage sludge acclimated to 2,4-dichlorophenol (2,4-DCP) and 3,4-dichlorophenol (3,4-DCP). The optimal temperature and pH for dechlorination of 2,4,6-trichlorophenol (2,4,6-TCP) were 30 degrees C and 7.2. Dechlorination of 2,4,6-TCP was inhibited by sulfate, nitrate, or ferric (III), but was increased by manganese (IV) except that product of 2,4-DCP was accumulated. Organic substrates such as pyruvate or acetate did not influence dechlorination of 2,4,6-TCP, but increased dechlorination of 2,4-DCP; nevertheless lactate showed no influence on dechlorination. When glucose was added as substrate, only 20% of 2,4,6-TCP dechlorination was found. Addition of methanogenesis inhibitor 2-bromoethane sulfonate did not influence dechlorination, but addition of eubacteria inhibitor vancomycin showed no dechlorination activity at the first few days, and finally complete transformation and accumulation of 2,4-DCP occurred. Because mixed culture was from Di-Hwa Wastewater Treatment Plant, adding the same water as culture medium was found to increase the dechlorination of 2,4,6-TCP and its product 2,4-DCP. Dechlorination by adding wastewater of Petroleum Corporation was also increased, especially for 2,4-DCP product, but only 7% of 2,4,6-TCP dechlorination was found after incubation with Hou-Chin river water for 14 days.
在本研究中，我们调查了适应2,4 - 二氯苯酚（2,4 - DCP）和3,4 - 二氯苯酚（3,4 - DCP）的城市污水污泥对氯酚进行还原脱氯的潜力。2,4,6 - 三氯苯酚（2,4,6 - TCP）脱氯的最佳温度和pH分别为30℃和7.2。2,4,6 - TCP的脱氯受到硫酸盐、硝酸盐或铁（III）的抑制，但除了2,4 - DCP产物积累外，锰（IV）可促进其脱氯。丙酮酸或乙酸盐等有机底物不影响2,4,6 - TCP的脱氯，但可增加2,4 - DCP的脱氯；然而，乳酸盐对脱氯没有影响。当添加葡萄糖作为底物时，仅发现20%的2,4,6 - TCP发生脱氯。添加产甲烷抑制剂2 - 溴乙烷磺酸盐不影响脱氯，但添加真细菌抑制剂万古霉素在最初几天没有脱氯活性，最终发生了2,4 - DCP的完全转化和积累。由于混合培养物来自东华污水处理厂，发现添加与培养基相同的水可增加2,4,6 - TCP及其产物2,4 - DCP的脱氯。添加石油公司的废水也可增加脱氯，特别是对于2,4 - DCP产物，但与后里河水孵育14天后，仅发现7%的2,4,6 - TCP发生脱氯。
