Bell R C, Khurana M, Ryan E A, Finegood D T
Department of Medicine, University of Alberta, Edmonton, Canada.
Endocrinology. 1994 Dec;135(6):2681-7. doi: 10.1210/endo.135.6.7988458.
Gender differences after treatment with streptozotocin (STZ) have been previously reported; however, differences in the glucose response to islet transplantation in STZ-induced diabetes in male and female rats after islet transplantation have not been examined. Male and female Wistar-Furth rats were made diabetic using STZ (55 mg/kg BW) and then given an intraportal islet transplant. Control animals received sham injections and sham transplant surgery; diabetic animals received STZ and sham surgery. In male animals, islet grafts contained 0 (diabetic), 250, 500, 1000, 1500, 2000, and 3000 islets; in female rats, grafts were made up of 0, 500 700, 750, 1000, or 2500 islets. STZ treatment had more dramatic effects on male than female rats. During the diabetic phase, body weights of male rats were significantly reduced compared to those of control male animals; this was not observed among females. Although all STZ-treated animals were hyperglycemic, plasma glucose levels in male diabetic rats were significantly higher than those in females during this phase (29.8 +/- 2.1 vs. 24.6 +/- 0.6 mM). After islet transplantation, body weight gain was positively associated with the number of islets transplanted in male rats (r2 = 0.59; P < 0.01), but not in females (r2 = 0.09; P > 0.8). In both male and female rats, animals that received 1000 islets or more were generally normoglycemic by 3 weeks posttransplant (males, 10.8 +/- 2.2 mM; females, 7.1 +/- 0.2 mM). Approximately 60% of male and female animals that received 500 islets achieved a reduction in plasma glucose levels. Mean plasma glucose levels were 17.2 +/- 2.3 in the females and 22.6 +/- 1.0 mM in males. However, a significantly larger proportion of female 500-islet animals (6 of 16) achieved a plasma glucose level of 9.5 mM or less compared with males receiving 500 islets (2 of 30). Multivariate regression analysis suggests that sex and islet number interact to affect glycemic normalization after islet transplantation. Gender differences appear to influence body weight and plasma glucose responses to islet transplantation. This finding may have particular relevance when a marginal number of functional islets are available.
先前已有关于链脲佐菌素（STZ）治疗后性别差异的报道；然而，对于雄性和雌性大鼠在STZ诱导的糖尿病胰岛移植后，其对胰岛移植的葡萄糖反应差异尚未进行研究。将雄性和雌性Wistar-Furth大鼠用STZ（55mg/kg体重）诱导成糖尿病，然后进行门静脉内胰岛移植。对照动物接受假注射和假移植手术；糖尿病动物接受STZ和假手术。在雄性动物中，胰岛移植包含0个（糖尿病组）、250个、500个、1000个、1500个、2000个和3000个胰岛；在雌性大鼠中，移植的胰岛由0个、500个、700个、750个、1000个或2500个胰岛组成。STZ治疗对雄性大鼠的影响比对雌性大鼠更显著。在糖尿病阶段，雄性大鼠的体重与对照雄性动物相比显著降低；雌性大鼠未观察到这种情况。尽管所有接受STZ治疗的动物均出现高血糖，但在此阶段雄性糖尿病大鼠的血浆葡萄糖水平显著高于雌性大鼠（29.8±2.1 vs. 24.6±0.6mM）。胰岛移植后，雄性大鼠体重增加与移植的胰岛数量呈正相关（r2 = 0.59；P < 0.01），而雌性大鼠则不然（r2 = 0.09；P > 0.8）。在雄性和雌性大鼠中，接受1000个或更多胰岛移植的动物在移植后3周时通常血糖正常（雄性，10.8±2.2mM；雌性，7.1±0.2mM）。接受500个胰岛移植的雄性和雌性动物中，约60%的动物血浆葡萄糖水平有所降低。雌性动物的平均血浆葡萄糖水平为1
