抗金属卟啉的单克隆抗体。与结构不同的金属卟啉相互作用的特异性。

Monoclonal antibodies against metalloporphyrins. Specificity of interaction with structurally different metalloporphyrins.

作者信息

Savitsky A P, Demcheva M V, Ponomarev G V

机构信息

A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow.

出版信息

FEBS Lett. 1994 Dec 5;355(3):314-6. doi: 10.1016/0014-5793(94)01221-0.

DOI:10.1016/0014-5793(94)01221-0

PMID:7988696

Abstract

Monoclonal antibodies against Pd-coproporphyrin I have been obtained. The antibody specificity for free as well as for conjugated Pd-coproporphyrin I is characterized. Affinity constants are estimated for 3 monoclonal antibodies effectively interacting with free Pd-coproporphyrin I. A comparative study on the binding of monoclonal antibodies with analogues and derivatives of Pd-coproporphyrin I has revealed that the antigen is mainly located inside the antibody paratope. The protein adjoins complementary to the metalloporphyrin in such a manner that antibodies obtained discern only isomer I, and to some degree, isomer III of coproporphyrin.

摘要

已获得针对钯-粪卟啉I的单克隆抗体。对游离及结合型钯-粪卟啉I的抗体特异性进行了表征。估算了3种与游离钯-粪卟啉I有效相互作用的单克隆抗体的亲和常数。对单克隆抗体与钯-粪卟啉I类似物及衍生物结合的比较研究表明，抗原主要位于抗体互补决定区内部。蛋白质以这样一种方式与金属卟啉互补结合，即所获得的抗体仅识别粪卟啉的异构体I，在一定程度上也识别异构体III。