Bernat A, Sainte-Marie M, Roque C, Ingelaere V, Maffrand J P, Herbert J M
Sanofi Recherche Haemobiology Research Department, Toulouse, France.
Haemostasis. 1994 Jul-Aug;24(4):209-18. doi: 10.1159/000217104.
Procoagulant activity of the endothelium and of monocytes was induced by an intravenous administration of a low dose of endotoxin in the rabbit. Venous thrombosis induced by a 45-min stasis of the jugular vein was dramatically increased after the administration of endotoxin. Endotoxin enhanced venous thrombosis in a dose-dependent manner which was the highest after the administration of 1 microgram/kg. This effect increased with time but was optimal when endotoxin was injected 4 h before stasis induction. Leukocytes appear to play a key role in this process since mechlorethamine-induced leukopenia totally inhibited thrombus formation. Hirudin and heparin, administered intravenously 5 min before stasis, were both potent inhibitors of endotoxin-induced venous thrombosis exhibiting ED50 values of 3.8 +/- 1.0 and 2.3 +/- 1.1 microgram/kg, respectively.
静脉注射低剂量内毒素可诱导兔内皮细胞和单核细胞的促凝血活性。颈静脉淤滞45分钟诱导的静脉血栓形成在内毒素给药后显著增加。内毒素以剂量依赖的方式增强静脉血栓形成，在给予1微克/千克后最高。这种作用随时间增加，但在内毒素在诱导淤滞前4小时注射时最为显著。白细胞似乎在这一过程中起关键作用，因为氮芥诱导的白细胞减少完全抑制了血栓形成。在淤滞前5分钟静脉注射水蛭素和肝素，二者都是内毒素诱导的静脉血栓形成的有效抑制剂，其半数有效剂量（ED50）分别为3.8±1.0和2.3±1.1微克/千克。
