Garcia J L
Ann Microbiol (Paris). 1976 Apr;127(3):401-14.
Nitric oxide gas evolution from nitrite was studied in vitro in three rice soils by gas chromatography. Autoclaved soils showed an NO evolution when supplemented with nitrite. Yet, when temperature of incubation, soil pH, soil moisture content and nitrite concentration were varied in the three soils, and with addition of nitrite reductase inhibitors, it appeared in one soil that NO production was partially a biological process. Thus, NO formation was two times as high in non-sterile soil as in sterile soil, and decreased when the temperature increased. Optimal NO production occurred at about neutrality and increased with increasing soil moisture content; moreover, this NO formation increased much less than in the other two soils with increasing nitrite concentration. Finally, the first soil contained three times more denitrifying bacteria tolerating a high nitrite concentration (5 g/1) that the other soils.
通过气相色谱法在三种水稻土中对亚硝酸盐产生一氧化氮气体的过程进行了体外研究。经高压灭菌的土壤在添加亚硝酸盐后会产生一氧化氮。然而，当改变三种土壤的培养温度、土壤pH值、土壤含水量和亚硝酸盐浓度，并添加亚硝酸还原酶抑制剂时，在一种土壤中发现一氧化氮的产生部分是一个生物过程。因此，非无菌土壤中一氧化氮的形成量是无菌土壤中的两倍，且随着温度升高而降低。一氧化氮的最佳产生量出现在接近中性的条件下，并随着土壤含水量的增加而增加；此外，随着亚硝酸盐浓度的增加，这种土壤中一氧化氮的形成量比其他两种土壤增加得少得多。最后，第一种土壤中耐高浓度亚硝酸盐（5克/升）的反硝化细菌数量是其他土壤的三倍。
