Garcia J L
Ann Microbiol (Paris). 1977 May-Jun;128A(4):433-46.
Dentrifying mesophilic and thermophilic bacteria were looked for in rice paddies of Senegal by culturing samples in medium with high concentrations of nitrate or nitrite (5 g/l) as respiratory substrate. These cultures revealed the existence of two populations. 1) a population of denitrifying mesophiles which tolerate high concentrations of nitrite, these organisms being mostly spore-formers and relatively numerous in these soils; growth studies showed them to be very diverse: a) "nitrite-dependent" bacteria unable to reduce NO-3; b) bacteria highly nitrite-tolerant with rapid growth on 5 g/l nitrite; c) bacteria slightly nitrite-tolerant with weak or no growth on more than 3 g/l nitrite; d) NO-tolerant bacteria which use nitric oxide as respiratory substrate for growth; e) N2O-deficient bacteria unable to grow on nitrous oxide; 2) a population of denitrifying thermophilic spore-formers which are numerous in some soils and tolerate nitrite more or less well. Measuring of the denitrifying activity of washed cells demonstrated that, in general, cells grown anaerobically on nitrite show much more activity than cells grown anaerobically on nitrate. This nitrite-tolerant population seems fairly heterogeneous, but it consists mostly of spore-forming species of the genus Bacillus.
通过在含有高浓度硝酸盐或亚硝酸盐（5克/升）作为呼吸底物的培养基中培养样品，在塞内加尔的稻田中寻找反硝化嗜温菌和嗜热菌。这些培养物揭示了两个菌群的存在。1）一群耐高浓度亚硝酸盐的反硝化嗜温菌，这些微生物大多是产芽孢菌，在这些土壤中数量相对较多；生长研究表明它们非常多样：a）“亚硝酸盐依赖型”细菌，不能还原NO₃⁻；b）对亚硝酸盐高度耐受，在5克/升亚硝酸盐上快速生长的细菌；c）对亚硝酸盐耐受性较弱，在超过3克/升亚硝酸盐上生长微弱或不生长的细菌；d）利用一氧化氮作为呼吸底物进行生长的耐NO细菌；e）不能在一氧化二氮上生长的N₂O缺陷型细菌；2）一群反硝化嗜热产芽孢菌，在一些土壤中数量众多，对亚硝酸盐的耐受性或多或少较好。对洗涤后的细胞进行反硝化活性测定表明，一般来说，在亚硝酸盐上厌氧生长的细胞比在硝酸盐上厌氧生长的细胞表现出更高的活性。这个耐亚硝酸盐的菌群似乎相当异质，但它主要由芽孢杆菌属的产芽孢物种组成。
