Ishisaki A, Oida S, Momose F, Amagasa T, Rikimaru K, Ichijo H, Sasaki S
Department of Oral and Maxillofacial Surgery, Tokyo Medical and Dental University, Japan.
Int J Cancer. 1994 Dec 15;59(6):783-8. doi: 10.1002/ijc.2910590613.
A human oral squamous-cell-carcinoma cell line, HOC313, was found to produce a factor which stimulates cell motility in an autocrine manner. The motility factor of HOC313 cells also promoted the locomotory activity of B16 murine melanoma cells reported to be sensitive to autocrine motility factor (AMF). HOC313 cells were found to express a large amount of AMF-receptor mRNA. In addition, the cell motility activity of HOC313 cells was completely blocked by pertussis toxin, a known inhibitor of AMF activity, suggesting that the motility factor of HOC313 cells may be AMF or a closely related factor. Immunocytochemical analysis has revealed that the AMF-like factor of HOC313 cells diminishes the cell-surface expression of adhesive molecule E-cadherin. These results suggest that down-regulation of E-cadherin may be involved in the cell-motility activity induced by the AMF-like factor of HOC313 cells.
人们发现一种人类口腔鳞状细胞癌细胞系HOC313能产生一种以自分泌方式刺激细胞运动的因子。HOC313细胞的运动因子还能促进据报道对自分泌运动因子（AMF）敏感的B16小鼠黑色素瘤细胞的运动活性。人们发现HOC313细胞表达大量的AMF受体mRNA。此外，HOC313细胞的细胞运动活性被百日咳毒素完全阻断，百日咳毒素是一种已知的AMF活性抑制剂，这表明HOC313细胞的运动因子可能是AMF或与之密切相关的因子。免疫细胞化学分析显示，HOC313细胞的AMF样因子会减少黏附分子E-钙黏蛋白的细胞表面表达。这些结果表明，E-钙黏蛋白的下调可能参与了HOC313细胞的AMF样因子诱导的细胞运动活性。
