黑曲霉NCIM-616β-半乳糖苷酶生产能力的提高。

Enhancement of beta-galactosidase productivity of Aspergillus niger NCIM-616.

作者信息

Rasouli I, Kulkarni P R

机构信息

Department of Chemical Technology, University of Bombay, India.

出版信息

J Appl Bacteriol. 1994 Oct;77(4):359-61. doi: 10.1111/j.1365-2672.1994.tb03435.x.

DOI:10.1111/j.1365-2672.1994.tb03435.x

PMID:7989263

Abstract

A series of mutations was carried out with Aspergillus niger NCIM-616 as the parent strain. A mutant strain NG-4 with 28% increased beta-galactosidase productivity was produced with N-methyl-N-nitro-N-nitrosoguanidine (NTG) at 1500 micrograms ml-1 concentration and exposure time of 60 min. This mutant yielded a third generation u.v.-treated strain, UV-5 with a 117.6% increase in beta-galactosidase productivity with respect to the parent strain.

摘要

以黑曲霉NCIM - 616作为亲本菌株进行了一系列突变实验。用浓度为1500微克/毫升的N - 甲基 - N - 硝基 - N - 亚硝基胍（NTG）处理60分钟，获得了β - 半乳糖苷酶产量提高28%的突变菌株NG - 4。该突变体又产生了第三代经紫外线处理的菌株UV - 5，其β - 半乳糖苷酶产量相对于亲本菌株提高了117.6%。