Okafor J I, Okunji P O
Department of Microbiology, University of Nigeria, Nsukka.
J Commun Dis. 1994 Jun;26(2):75-81.
A total of 413 soft, loose or watery stool specimens from patients with acute diarrhoea were screened for presence of Cryptosporidium oocysts. Safranin-methylene blue staining technique was used for detecting Cryptosporidium oocysts in the stool samples. The oocysts were identified in 52 (12.5 per cent) of the samples, while 69 samples (16.7 per cent) showed mixed infections. Children between the ages of 2 and 15 years were most infected by this parasite while infection occurred more in females than males for all age groups. These findings indicate that Cryptosporidium is an important etiologic agent of diarrhoea. Hence there is need to include its identification in laboratory analysis of diarrhoeal stool samples.
对413份急性腹泻患者的软便、稀便或水样便标本进行了隐孢子虫卵囊检测。采用番红 - 亚甲蓝染色技术检测粪便样本中的隐孢子虫卵囊。在52份(12.5%)样本中发现了卵囊,69份(16.7%)样本显示混合感染。2至15岁的儿童受这种寄生虫感染最为严重,且在所有年龄组中女性感染多于男性。这些发现表明隐孢子虫是腹泻的重要病原体。因此,有必要在腹泻粪便样本的实验室分析中进行其鉴定。