Gieryng R, Prost M, Gieryng H, Mazur T, Kasprzak W
Katedry Biologii i Parazytologii AM, Lublinie.
Klin Oczna. 1994 Apr-May;96(4-5):163-7.
In seventeen patients who suffered of corneal ulcer, and were negative in bacteriological and mycological examinations, parasitological tests were performed between 1990-1992. Acanthamoeba keratitis was diagnosed in one patient with characteristic corneal changes of right eye following trauma. The patient was not a contact lens wearer. The amoeba found in direct smears of scraping from corneal ulcer and isolated and cloned in in vitro conditions (isolate-clone-No. 2961) belonged to Acanthamoeba polyphaga species. The trophozoites showed a low degree of invasiveness and high virulence when tested in BALB/c mice. The authors discussed the epidemiology of Acanthamoeba keratitis and pointed out to the necessity of accurate laboratory diagnosis and discerning interpretation of the results to prevent an Acanthamoeba-phobia among the potential patients and most of all among the medical staff.
1990年至1992年间，对17例角膜溃疡患者进行了寄生虫学检测，这些患者的细菌学和真菌学检查均为阴性。一名患者在右眼外伤后出现典型角膜病变，被诊断为棘阿米巴角膜炎。该患者并非隐形眼镜佩戴者。从角膜溃疡刮片中直接涂片发现的阿米巴，在体外条件下进行了分离和克隆（分离株-克隆株-编号2961），属于多食棘阿米巴种。滋养体在BALB/c小鼠中测试时显示出低侵袭性和高毒力。作者讨论了棘阿米巴角膜炎的流行病学，并指出准确的实验室诊断和对结果的审慎解读对于防止潜在患者尤其是医务人员中出现棘阿米巴恐惧症的必要性。
