Wegerhoff R, Breidbach O
Institut für Angewandte Zoologie, Bonn, Germany.
J Neurosci Methods. 1994 Jul;53(1):87-93. doi: 10.1016/0165-0270(94)90148-1.
Our method combines intracellular dye injection and immunohistochemistry. Under optical control, Lucifer Yellow was injected into immunohistochemically identified neurons that reside in fixed tissue. The technique allows visualization of the complete arborization patterns of immunostained neurons. Injections were performed on small neurons (somata < 10 microns in diameter). The technique works on microslices of insect brain. Standard immunohistochemical procedures have only been varied slightly, omitting Triton X-100 treatment. Anti-Lucifer Yellow immunohistochemistry, or alternatively the photoconversion technique, enables extension of the morphological analysis of these cells to the electron microscopic level. In the present study, Lucifer Yellow injections were performed on immunohistochemically pretreated brain microslices (anti-Locusta tachykinin II antiserum) of the beetle Tenebrio molitor.
我们的方法结合了细胞内染料注射和免疫组织化学。在光学控制下，将荧光黄注入固定组织中经免疫组织化学鉴定的神经元。该技术能够观察到免疫染色神经元完整的树突分支模式。注射操作针对小神经元（胞体直径<10微米）进行。该技术适用于昆虫脑的微切片。标准免疫组织化学程序仅略有改动，省略了Triton X-100处理。抗荧光黄免疫组织化学，或者光转化技术，能够将这些细胞的形态学分析扩展到电子显微镜水平。在本研究中，对甲虫黄粉虫经免疫组织化学预处理的脑微切片（抗黄粉虫速激肽II抗血清）进行了荧光黄注射。
