Tsiuriupa G P, Pashkov V N
Mol Biol (Mosk). 1994 Jul-Aug;28(4):725-37.
A review is presented of the possibilities of using Xenopus oocytes for molecular-biological studies. Combination of biochemical, electrophysiological, and molecular-genetic methods applied to a single oocyte provides exceptional conditions for getting information about the biogenesis, structure function relationships, and modulation of plasma membrane proteins. These investigations are provided for by the oocyte ability to efficiently transcribe and translate foreign genetic information, to correctly process nascent polypeptides to target them to the proper subcellular compartment, and to couple them to the oocyte signaling pathways.
本文综述了利用非洲爪蟾卵母细胞进行分子生物学研究的可能性。将生化、电生理和分子遗传学方法结合应用于单个卵母细胞,为获取有关质膜蛋白的生物发生、结构功能关系及调节的信息提供了绝佳条件。卵母细胞高效转录和翻译外源遗传信息、正确加工新生多肽使其靶向至合适的亚细胞区室并将它们与卵母细胞信号通路偶联的能力,使得这些研究得以开展。