Kawaguchi M, Kawashima F, Ohshima K, Kawaguchi S, Wada H
Department of Pediatrics, Hyogo College of Medicine, Nishinomiya, Japan.
Nephron. 1994;68(1):97-103. doi: 10.1159/000188226.
The renal epithelium appears to be an important target tissue for retinoic acid and epidermal growth factor (EGF). We report here that retinoic acid enhances the proliferative effect of EGF on glomerular epithelial cells (GEC) in vitro and also increases EGF binding to GEC. When GEC were exposed to EGF (> or = 1 ng/ml), cellular DNA synthesis was markedly increased. Moreover, the stimulating effect of EGF was synergistically increased by retinoic acid at 5 micrograms/ml. 125I-EGF binding to cultured GEC was increased approximately 3-fold after addition of retinoic acid to cultures for 48 h. Analysis of 125I-EGF binding revealed 8.1 x 10(4) receptors per control (untreated) cell, while retinoic acid-treated cells demonstrated an increase to 14.3 x 10(4) receptors per cell with no detectable change in receptor affinity. These findings suggest that interactions between retinoic acid and EGF may play an important role in the regulation of GEC growth.
肾上皮似乎是视黄酸和表皮生长因子（EGF）的重要靶组织。我们在此报告，视黄酸在体外增强了EGF对肾小球上皮细胞（GEC）的增殖作用，并且还增加了EGF与GEC的结合。当GEC暴露于EGF（≥1 ng/ml）时，细胞DNA合成明显增加。此外，5微克/毫升的视黄酸协同增强了EGF的刺激作用。在培养物中加入视黄酸48小时后，125I-EGF与培养的GEC的结合增加了约3倍。对125I-EGF结合的分析显示，每个对照（未处理）细胞有8.1×10⁴个受体，而视黄酸处理的细胞显示每个细胞的受体增加到14.3×10⁴个，受体亲和力没有可检测到的变化。这些发现表明，视黄酸和EGF之间的相互作用可能在GEC生长的调节中起重要作用。
