在离体兔肠道制备物中自发记录以及由胃动素和红霉素诱导产生的移行性运动复合波。

Migrating motor complex recorded spontaneously and induced by motilin and erythromycin in an ex vivo rabbit intestinal preparation.

作者信息

Marzio L, Grossi L, Martelli L, Falcucci M, Lapenna D

机构信息

Istituto di Fisiopatologia Medica, G.D'Annunzio University, Chieti, Italy.

出版信息

Peptides. 1994;15(6):1067-77. doi: 10.1016/0196-9781(94)90072-8.

DOI:10.1016/0196-9781(94)90072-8

PMID:7991450

Abstract

We investigated basal motility and the motor effects of motilin, erythromycin, and prostigmine on segments of rabbit gastrointestinal tract removed from extrinsic neural and vascular pathway and immersed in an oxygenated organ bath. Motility was recorded by means of four strain gauges sutured on the serosal surface of the segment. During basal recording, clusters of duodenal contractions that propagated distally, resembling phase III activity of migrating motor complex, were seen. Motilin (10(-6) M) and erythromycin (10(-6) M) induced a propagated cluster of contractions similar to the phase III recorded during the basal period. Prostigmine (10(-6) M) induced a simultaneous increase in gastric and small intestinal motility. Atropine (10(-5) M) prevented the motor effect of motilin, erythromycin, and prostigmine. Thus, MMCs do not appear to require central input for initiation and propagation. Motilin and erythromycin stimulate MMCs through an enteric cholinergic mechanism; therefore, the previously reported smooth muscle receptors for both substances were not apparent in the ex vivo preparation.

摘要

我们研究了基础运动性以及胃动素、红霉素和新斯的明对从兔胃肠道分离出的、去除了外在神经和血管通路并浸浴于充氧器官浴中的肠段的运动效应。通过缝合在肠段浆膜表面的四个应变片记录运动性。在基础记录期间，观察到十二指肠收缩簇向远端传播，类似于移行运动复合波的Ⅲ期活动。胃动素（10⁻⁶ M）和红霉素（10⁻⁶ M）诱导出与基础期记录的Ⅲ期相似的传播性收缩簇。新斯的明（10⁻⁶ M）诱导胃和小肠运动性同时增加。阿托品（10⁻⁵ M）可阻断胃动素、红霉素和新斯的明的运动效应。因此，移行运动复合波的起始和传播似乎并不需要中枢输入。胃动素和红霉素通过肠胆碱能机制刺激移行运动复合波；因此，先前报道的这两种物质的平滑肌受体在离体标本中并不明显。