Mercado-Blanco J, Olivares J
Departamento de Microbiología, Estación Experimental del zaidín, CSIC, Granada, Spain.
Plasmid. 1994 Jul;32(1):75-9. doi: 10.1006/plas.1994.1046.
The large plasmid, pRmeGR4a, of Rhizobium meliloti GR4 is a nonsymbiotic, self-transmissible replicon that shows a high degree of stability. Plasmid replication and stabilization functions are located on a 4.8-kb PstI fragment. Analysis of the nucleotide sequence of this DNA region revealed the presence of six open reading frames (ORFs) with coding capabilities; all are transcribed in the same direction. Only one of the ORFs (ORF3) seems to be essential for replication. Its predicted protein sequence shows homology with RepC, a protein that has been suggested to be involved in the replication of Agrobacterium Ri and Ti plasmids.
苜蓿根瘤菌GR4的大质粒pRmeGR4a是一个非共生、可自我转移的复制子，具有高度稳定性。质粒复制和稳定功能位于一个4.8kb的PstI片段上。对该DNA区域核苷酸序列的分析揭示了六个具有编码能力的开放阅读框（ORF）；所有这些开放阅读框都朝同一方向转录。其中只有一个开放阅读框（ORF3）似乎对复制至关重要。其预测的蛋白质序列与RepC具有同源性，RepC是一种被认为参与根癌农杆菌Ri和Ti质粒复制的蛋白质。
