Goto S, Kamada N, Lord R, Kobayashi E, Enosawa S, Kim Y L
Department of Surgery, University of Queensland, Herston, Australia.
Transplantation. 1994 Dec 15;58(11):1230-5.
Immunological aspects after orthotopic rat liver retransplantation (re-OLT) were examined in association with cell migration and mixed chimerism. At day 2 after the first orthotopic liver transplantation (day 0) in the combination of DA (MHC haplotype, RT1a) donor into PVG (RT1c) recipient, the grafted DA liver was removed and a new PVG liver was implanted into the same PVG recipient (re-OLT). In the PVG recipient at various times after the re-OLT, DA-derived antigen and cells were detected using a DA-specific anti-class I mAb R3/13 in conjunction with ELISA, immunoblotting, and immunohistochemistry. The level of soluble class I antigen, which had risen to 270 ng/ml after the first OLT, substantially decreased within 24 hr after re-OLT. Using immunoblotting, DA class I antigen was detected in the PVG recipient's lymphoid organs at day 3 after DA liver grafting and persisted for up to 21 days after the DA liver was replaced by a new PVG liver. Immunohistochemistry on sections of spleen from re-OLT rats showed that the level of migratory cells expressing DA class I correlated with the findings obtained by immunoblotting. While the DA-derived antigen and cells were detected in the re-OLT recipient, the DA-specific inhibition of mixed lymphocyte reaction was observed in re-OLT serum. Our results suggest that the implanted DA liver graft was the source of DA soluble class I antigen, but DA-derived antigen and cells detected in the re-OLT recipient organs could persist for a relatively long time under immunosuppression after the implanted DA liver was removed by re-OLT.
结合细胞迁移和混合嵌合现象，对大鼠原位肝再次移植（re-OLT）后的免疫学方面进行了研究。在DA（MHC单倍型，RT1a）供体与PVG（RT1c）受体组合的首次原位肝移植（第0天）后第2天，切除移植的DA肝，并将新的PVG肝植入同一PVG受体（re-OLT）。在re-OLT后的不同时间点，使用DA特异性抗I类单克隆抗体R3/13结合ELISA、免疫印迹和免疫组织化学检测PVG受体中DA来源的抗原和细胞。可溶性I类抗原水平在首次OLT后升至270 ng/ml，在re-OLT后24小时内大幅下降。使用免疫印迹法，在DA肝移植后第3天在PVG受体的淋巴器官中检测到DA I类抗原，并且在新的PVG肝取代DA肝后持续长达21天。对re-OLT大鼠脾脏切片进行免疫组织化学分析表明，表达DA I类的迁移细胞水平与免疫印迹结果相关。虽然在re-OLT受体中检测到DA来源的抗原和细胞，但在re-OLT血清中观察到DA对混合淋巴细胞反应的特异性抑制。我们的结果表明，植入的DA肝移植物是DA可溶性I类抗原的来源，但在re-OLT受体器官中检测到的DA来源的抗原和细胞在re-OLT切除植入的DA肝后，在免疫抑制下可能会持续相对较长的时间。
