Krenger W, Snyder K, Smith S, Ferrara J L
Department of Pediatric Oncology, Dana Farber Cancer Institute, Boston, Massachusetts 02115.
Transplantation. 1994 Dec 15;58(11):1251-7.
IL-10 is a regulatory cytokine of both T cells and monocytes. We have investigated the ability of IL-10 to regulate responses to alloantigens in vitro and in vivo. Addition of IL-10 to mixed lymphocyte cultures profoundly decreased the proliferation and IL-2 production by donor B10.BR cells stimulated with CBA cells expressing minor histocompatibility antigens. Administration of IL-10 for a period of 2 weeks after bone marrow transplantation decreased the expansion of CD4+ and CD8+ donor T cells. In addition, splenocytes from BMT mice treated with IL-10 secreted less IFN-gamma after stimulation with Con A in vitro. The suppression of the mitogen-driven proliferative response of lymphocytes from the IL-10-treated group could also be reversed with significantly less anti-IFN-gamma antibody than for saline-treated controls. However, treatment with IL-10 was not sufficient to alter significantly the clinical course of graft-versus-host disease in CBA recipient mice as assessed by survival, weight loss, and splenomegaly. The results suggest that exogenous IL-10 suppresses the afferent Th1 response in a graft-versus-host reaction but does not significantly diminish the effector stage of graft-versus-host disease.
白细胞介素-10是一种对T细胞和单核细胞均有调节作用的细胞因子。我们研究了白细胞介素-10在体外和体内调节同种异体抗原反应的能力。向混合淋巴细胞培养物中添加白细胞介素-10，可显著降低表达次要组织相容性抗原的CBA细胞刺激的供体B10.BR细胞的增殖及白细胞介素-2的产生。骨髓移植后给予白细胞介素-10 2周，可减少CD4⁺和CD8⁺供体T细胞的扩增。此外，用白细胞介素-10处理的骨髓移植小鼠的脾细胞在体外经刀豆蛋白A刺激后分泌的γ干扰素较少。与盐水处理的对照组相比，用明显较少的抗γ干扰素抗体即可逆转白细胞介素-10处理组淋巴细胞有丝分裂原驱动的增殖反应的抑制。然而，通过生存、体重减轻和脾肿大评估，白细胞介素-10治疗不足以显著改变CBA受体小鼠移植物抗宿主病的临床病程。结果表明，外源性白细胞介素-10在移植物抗宿主反应中抑制传入性Th1反应，但不会显著减轻移植物抗宿主病的效应阶段。
