Karpova L A, Belyĭ Iu F, Tartakovskiĭ I S, Prozorovskiĭ S V
Zh Mikrobiol Epidemiol Immunobiol. 1994 Jul-Aug(4):3-7.
A scheme of the purification of listeriolysin O produced by L. monocytogenes strain NCTC 7973 was developed. The isolation procedure included the cultivation of the bacteria in heart-brain broth, the concentration of culture liquid free of bacteria with ammonium sulfate, cation exchange chromatography on a column packed with CM-Sepharose and Mono S, gel chromatography on a column packed with Superose 12. The preparation obtained with the use of this procedure was homogeneous, as confirmed by the data of SDS electrophoresis. The protein obtained in this investigation was no different from the protein studied earlier in its physico-chemical properties (molecular weight, heat stability, inhibition with thiole-active compositions, cholesterol, sensitivity to proteolytic enzymes) and corresponded to the characteristics of thiole-dependent hemolysins.
开发了一种用于纯化单核细胞增生李斯特菌NCTC 7973菌株产生的李斯特菌溶血素O的方案。分离程序包括在脑心浸液肉汤中培养细菌，用硫酸铵浓缩无细菌的培养液，在填充有CM-琼脂糖凝胶和Mono S的柱上进行阳离子交换色谱，在填充有Superose 12的柱上进行凝胶色谱。如SDS电泳数据所证实，使用该程序获得的制剂是均一的。本研究中获得的蛋白质在其物理化学性质（分子量、热稳定性、硫醇活性成分抑制、胆固醇、对蛋白水解酶的敏感性）方面与早期研究的蛋白质没有差异，并且符合硫醇依赖性溶血素的特征。
