用微分脉冲极谱法测定人血清中乳酸脱氢酶活性的灵敏方法。
Sensitive method for measuring lactate dehydrogenase activity in human serum by differential-pulse polarography.
作者信息
Sulaiman S T, al-Najafi T S, Hamdon H S
机构信息
Department of Chemistry, College of Science, University of Mosul, Iraq.
出版信息
Analyst. 1994 Oct;119(10):2199-200. doi: 10.1039/an9941902199.
PMID:7992878
Abstract
A sensitive and accurate differential-pulse polarographic method is reported for the measurement of lactate dehydrogenase (LDH) activity. The method is based on the polarographic measurement of the NAD+ formed via the enzymic reaction of LDH with pyruvate and NADH. The method was successfully applied to measure LDH activity in the serum of normal and diseased individuals.
摘要
报道了一种灵敏且准确的差分脉冲极谱法用于测定乳酸脱氢酶（LDH）活性。该方法基于通过LDH与丙酮酸和NADH的酶促反应形成的NAD⁺的极谱测量。该方法已成功应用于测量正常人和病人血清中的LDH活性。
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