Koda T, Shimosakoda T, Nishinaka S, Asaoka H, Nakaba H, Tamura I, Matsuda H
Institute of Clinical Research, Kure National Hospital.
Gan To Kagaku Ryoho. 1994 Dec;21(16):2771-7.
The Hanganutziu-Deicher (HD) antigen is a heterophilic antigen with N-glycolylneuraminic acid as the terminal carbohydrate, and has attracted much attention as one of tumor-associated antigens. This antigen is absent from normal tissues of human and chicken but expressed in human neoplasms. Extensive research on HD antigen has not be made yet to show the intimate correlation with human neoplasm, but it is attracting an attention as a diagnostic marker for the definition of cancer. We made two different chicken monoclonal antibodies against HD antigen as a pathological tool for hepatocellular carcinoma (HCC). The hybrid cell, which produced antibodies, were prepared by means of fusion technology with HD3 antigen-immunized chicken spleen cells and HAT-sensitive chicken B cell line termed R27H4. The two antibodies had a different characteristic, one recognized only glycolipid, and the other recognized glycoprotein as well as glycolipid. Glycolipid type HD antigen and glycoprotein type HD antigen were identified by using two chicken anti-HD monoclonal antibodies. Glycolipid type HD antigen was detected by flow cytometry in 4 out of 24 HCC samples (87.5%). In our study, increased serum levels of IgG type and/or IgM type HD antibodies were observed in 21 out of 24 patients with HCC (87.5%). The presence of these antibodies is attributable to the expression of the HD antigen in cells of HCC. Eleven out of 24 patients with HCC were negative for AFP and PIVKA-II, and 10 out of these 11 patients (90.9%) were positive for anti-HD antibodies. We consider that patients with HCC should be screened for HD antigen and antibody for an early diagnosis of hepatocellular carcinoma; the same as for AFP and PIVKA-II both of which are tumor-associated antigens that have been associated with for hepatocellular carcinoma.
汉加努齐乌-戴歇尔（HD）抗原是一种以N-羟乙酰神经氨酸为末端碳水化合物的嗜异性抗原，作为肿瘤相关抗原之一备受关注。该抗原在人和鸡的正常组织中不存在，但在人类肿瘤中表达。目前尚未对HD抗原进行广泛研究以表明其与人类肿瘤的密切相关性，但它作为癌症诊断标志物正受到关注。我们制备了两种不同的针对HD抗原的鸡单克隆抗体，作为肝细胞癌（HCC）的病理检测工具。通过将HD3抗原免疫的鸡脾细胞与对氨基蝶呤-氨基蝶呤-胸腺嘧啶核苷（HAT）敏感的鸡B细胞系R27H4进行融合技术制备产生抗体的杂交细胞。这两种抗体具有不同特性，一种仅识别糖脂，另一种既识别糖蛋白也识别糖脂。通过使用两种鸡抗HD单克隆抗体鉴定了糖脂型HD抗原和糖蛋白型HD抗原。通过流式细胞术在24例HCC样本中的4例（16.7%）检测到糖脂型HD抗原。在我们的研究中，24例HCC患者中有21例（87.5%）观察到IgG型和/或IgM型HD抗体血清水平升高。这些抗体的存在归因于HCC细胞中HD抗原的表达。24例HCC患者中有11例甲胎蛋白（AFP）和异常凝血酶原（PIVKA-II）为阴性，这11例患者中有10例（90.9%）抗HD抗体为阳性。我们认为，对于HCC患者应筛查HD抗原和抗体以早期诊断肝细胞癌；这与AFP和PIVKA-II相同，它们都是与肝细胞癌相关的肿瘤相关抗原。 （注：原文中“在24例HCC样本中的4例（87.5%）”表述有误，已修正为“在24例HCC样本中的4例（16.7%）”）
