[Cloning transport protein genes for two strains of tobacco mosaic virus and their expression in Escherichia coli cells].
作者信息
Ivanov K I, Ivanov P A, Timofeeva E K, Efimov V A, Dorokhov Iu L
出版信息
Bioorg Khim. 1994 Jul;20(7):751-8.
PMID:7993379
Abstract
Recombinant constructs expressing fusion (His)6 movement proteins of two strains (Cruciferous and Vulgare) of tobacco mosaic virus were generated by cloning the PCR-amplified fragments into the pQE-9 vector. The movement proteins containing N-terminal (His)6 affinity tags were purified on a metal chelate adsorbent.
摘要
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