Tang C M, Margulis M, Shi Q Y, Fielding A
Department of Neurology, University of Maryland School of Medicine, Baltimore 21201.
Neuron. 1994 Dec;13(6):1385-93. doi: 10.1016/0896-6273(94)90423-5.
Miniature excitatory postsynaptic currents (mEPSCs) were elicited from small numbers of release sites after brief microperfusion of Ba2+ and K+ onto proximal dendritic processes of hippocampal neurons in culture. Temporal summation of closely timed mEPSCs deviated significantly from linearity. The number of instances of closely timed mEPSCs that were also closely matched in terms of peak amplitudes was significantly greater than that expected by chance. Amplitude pairing became statistically more significant after prolongation of mEPSC duration and inhibition of glutamate receptor desensitization with cyclothiazide. These results are best explained by postsynaptic receptors that approach saturation after quantal release of transmitter.
在体外培养的海马神经元近端树突过程中，短暂微量灌注Ba2+和K+后，从少数释放位点诱发微小兴奋性突触后电流（mEPSCs）。紧密定时的mEPSCs的时间总和显著偏离线性。在峰值幅度方面也紧密匹配的紧密定时的mEPSCs实例数量显著大于随机预期的数量。在用环噻嗪延长mEPSC持续时间并抑制谷氨酸受体脱敏后，幅度配对在统计学上变得更显著。这些结果最好用递质量子释放后接近饱和的突触后受体来解释。
